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KMID : 1145120180160010103
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Asian Journal of Beauty and Cosmetology
2018 Volume.16 No. 1 p.103 ~ p.112
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Inhibition of Apoptosis and Anti-inflammatory Effects of Embelin
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Yong Hee-Jung
Kim Gyu-Ri
Ahn Jin-Jung
An In-Sook
Kim Young-Sam
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Abstract
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Purpose: Embelin is a major active ingredient of Embelia ribes Burm, belonging to Myrsinaceae, and an important traditional medicinal plant of Indian origin. Embelin has been studied for anti-cancer, anti-inflammatory, and anti-oxidative effects. However, there have been no studies on the use of cosmetic raw materials for embelin. Therefore, in this study, we investigated the inhibition of apoptosis and the anti-inflammatory effect to confirm the possibility of embelin as a cosmetic material.
Methods: Cell viability was measured by using water-soluble tetrazolium salt (WST-1) assay. Quantitative real time polymerase chain reaction (qRT-PCR) was performed to quantitatively analyze the gene expression patterns of embelin-induced inhibition of apoptosis and anti-inflammatory effects in human dermal fibroblasts (HDFs). Apoptosis enzyme linked immunosorbent assay (ELISA) kit was used to confirm the degree of apoptosis. 5,5', 6,6'-tetrachloro-1,1', 3,3'-tetraethylbenzimidazolylcarbo cyanine iodide (JC-1) mitochondrial membrane potential detection kit was used to measure mitochondrial membrane potential.
Results: The WST-1 assay confirmed that the reduced cell viability by hydrogen peroxide (H2O2) was restored by embelin in a concentration-dependent manner. H2O2-induced apoptosis was restored by embelin, Bcl-2-associated X protein (BAX) gene expression was decreased and mitochondrial membrane potential difference was increased in a concentration dependent manner. It was confirmed that embelin inhibits apoptosis in HDFs treated with H2O2. In order to investigate the anti-inflammatory effect of embelin in H2O2-treated human dermal fibroblasts, gene expression of cyclooxygenase 2 (COX2), tumor necrosis factor α (TNFα), and interleukin 6 (IL6), which induce inflammation, was examined and found to be decreased by embelin in a concentration dependent manner.
Conclusion: These results suggest that embelin has a potential as an anti-aging cosmetic ingredient with inhibition of apoptosis and anti-inflammatory properties.
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KEYWORD
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Embelin, Anti-oxidative, Anti-inflammatory, Anti-aging, Human dermal fibroblasts
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